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CelLytic™ NuCLEAR™ Extraction Kit. SIGMA/NXTRACT – For mammalian tissue or cultured cells. Product Type: Chemical. Application A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose. CelLytic NuCLEAR Extraction Kit Product Code NXTRACT TECHNICAL BULLETIN Product Description The preparation of an extract from nuclei is often the first.

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TECHNICAL BULLETIN. CelLytic NuCLEAR Extraction Kit. Product Code NXTRACT – PDF

Get customized pricing for your organization when available. Join Our Mailing List! Oct-1 Motif Preparation Method: Gel Shift Assay of Oct Two different methods, described nucleratm the protocol were utilized: Method 1 used NP detergent; Method 2 relied on passages through a syringe instead of a detergent to extract the nuclei.

Purity of Cytoplasmic and Nuclear Proteins. Properties Descriptions Safety Info.

NXTRACT kit was used to study the impact of nufleartm on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice.

It was also used to test the therapeutic potential of andrographolide for treating endometriosis. The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. CelLytic is a trademark of Sigma-Aldrich Co. Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells.


TECHNICAL BULLETIN. CelLytic NuCLEAR Extraction Kit. Product Code NXTRACT

All reagents necessary for extraction are included. Select a Favorite List. Please fill out the form below if you want to request literature from Krackeler Scientific. Add multiple items to your shopping cart by entering the Krackeler Cat.

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